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the etiological agent of Lyme borreliosis. The results indicate that there were no significant differences between the identified microorganisms associated with. Denaturing gradient gel electrophoresis was performed in a V20 CDC Dual Vertical Unit System (Scie-Plas, Gainsborough Warwickshire, UK) at 60C/20 V for 10 min and then at 60C/60 V for 16 hr.5x TAE as previously described. Were present in a large number of ticks from groups G1 and.

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