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Dgge thesis

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the etiological agent of Lyme borreliosis. The results indicate that there were no significant differences between the identified microorganisms associated with. Denaturing gradient gel electrophoresis was performed in a V20 CDC Dual Vertical Unit System (Scie-Plas, Gainsborough Warwickshire, UK) at 60C/20 V for 10 min and then at 60C/60 V for 16 hr.5x TAE as previously described. Were present in a large number of ticks from groups G1 and.

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A scatter plot was created to study the distribution of bacterial species between emily dickinson essay on death the three groups. Pseudomonas putida, Spiroplasma spp., Roseomonas spp., Methylovirgula spp., Methylobacterium spp., Erwinia billingiae, Raoultella spp., and Enterobacter spp. Other than the known tick-borne pathogens. Species are identified in the figure with a number corresponding to the species number in Table. The calculated difference within each group gave a variance ratio.905. Statistical analysis was performed to analyse the variance between the microbial contents from groups G1, G2, and. The amplified fragments are separated in a gradient polyacrylamide gel with buffer heated to 60C. Copyright 2013 Ann-Kristin Tveten. Table 3: One-way anova analysis of the coherence between groupings of microbial communities and between species. Statistically, there are no specific microbial profiles that can be used to describe group. Burgdorferi sensu lato-.

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